Thomas-group

Raman spectroscopy

 

From its inception in 1837 haemotoxylin and eosin (H&E) staining has been the primary contrasting technique for clinical pathology and tissue-based cancer diagnostics. However, despite enhancements such as immunohistochemistry, the technique is essentially qualitative and so suffers from inter and intra-observer variability. Raman spectroscopy can help transform pathology into a quantitative technique. It is a technology that can detect the change in wavelength from incident photons after being scattered by a biological sample. This change in wavelength is unique to particular chemical species, giving rise to the possibility of detecting hitherto unseen biochemical changes. The challenge lies in a thorough mathematical treatment to make sense of rich spectral datasets in a way which is meaningful to clinicians.

People: Riana Gaifulina,
           Nathan Blake

Collaborators: Lewis Griffin, UCL
               Dr Manuel Rodriguez-Justo, UCLH
               Prof Nick Stone, Exeter
               Dr Catherine Kendall, GRH
               Dr Gavin Lloyd, GRH
               Dr Aaran Lewis, University of Gothenburg
               Renishaw PLC
               Roche Ltd

Publications:
Gaifulina, R., Maher, A.T., Kendall, C., Nelson, J., Rodriguez-Justo, M., Lau, K. and Thomas, G,M. (2016)
Label-free Raman spectroscopic imaging to extract morphological and chemical information from a formalin-fixed, paraffin-embedded rat colon tissue section.
International Journal of  Experimental Pathology. 97(4):337-350

Lewis, A. T., Gaifulina, R., Isabelle, M., Dorney, J., Woods, M. L., Lloyd, G. R., Lau, K., Rodriguez-Justo, M., Kendall, C., Stone, N., and Thomas, G. M. (2016)
Mirrored stainless steel substrate provides improved signal for Raman spectroscopy of tissue and cells.
J. Raman Spectrosc., doi: 10.1002/jrs.4980.

Gaifulina, R., Lau, K., Rodriguez-Justo, M., Kendall, C. and Thomas, G.M. (2014)
Light at the End of the Tunnel: Application of Raman Spectroscopy in Colorectal Cancer Diagnostics.
Austin Journal of Clinical Pathology. 1(4) pp. 1-3

This page was last modified on 06 Jan 2017.