Raman spectroscopy


From its inception in 1837 haemotoxylin and eosin (H&E) staining has been the primary contrasting technique for clinical pathology and tissue-based cancer diagnostics. However, despite enhancements such as immunohistochemistry, the technique is essentially qualitative and so suffers from inter and intra-observer variability. Raman spectroscopy can help transform pathology into a quantitative technique. It is a technology that can detect the change in wavelength from incident photons after being scattered by a biological sample. This change in wavelength is unique to particular chemical species, giving rise to the possibility of detecting hitherto unseen biochemical changes. The challenge lies in a thorough mathematical treatment to make sense of rich spectral datasets in a way which is meaningful to clinicians.

People: Riana Gaifulina,
           Nathan Blake

Collaborators: Lewis Griffin, UCL
               Dr Manuel Rodriguez-Justo, UCLH
               Prof Nick Stone, Exeter
               Dr Catherine Kendall, GRH
               Dr Gavin Lloyd, GRH
               Dr Aaran Lewis, University of Gothenburg
               Renishaw PLC
               Roche Ltd

Gaifulina, R., Maher, A.T., Kendall, C., Nelson, J., Rodriguez-Justo, M., Lau, K. and Thomas, G,M. (2016)
Label-free Raman spectroscopic imaging to extract morphological and chemical information from a formalin-fixed, paraffin-embedded rat colon tissue section.
International Journal of  Experimental Pathology. 97(4):337-350

Lewis, A. T., Gaifulina, R., Isabelle, M., Dorney, J., Woods, M. L., Lloyd, G. R., Lau, K., Rodriguez-Justo, M., Kendall, C., Stone, N., and Thomas, G. M. (2016)
Mirrored stainless steel substrate provides improved signal for Raman spectroscopy of tissue and cells.
J. Raman Spectrosc., doi: 10.1002/jrs.4980.

Gaifulina, R., Lau, K., Rodriguez-Justo, M., Kendall, C. and Thomas, G.M. (2014)
Light at the End of the Tunnel: Application of Raman Spectroscopy in Colorectal Cancer Diagnostics.
Austin Journal of Clinical Pathology. 1(4) pp. 1-3

This page was last modified on 06 Jan 2017.